Due to their safety, high expression levels, and post-translational modification capabilities, baculovirus expression vectors have become a widely used tool for producing recombinant proteins in various fields, including biotechnology, pharmacology, and vaccine development. The core of baculovirus expression system are the vector as well as viral DNA. The key function of vector is to carry foreign gene, mediating the integration of foreign gene and baculovirus gene into infectious nucleic acid sequence, and finally realize the expression of foreign gene. Each vector or viral DNA exhibits unique features, by which one expression approach can be distinguished from another.
Baculovirus expression vectors are constructed by replacing the viral genes encoding for structural proteins with the gene of interest, resulting in the production of the recombinant protein in infected insect cells. The most commonly used baculovirus expression vector is derived from the Autographa californica nucleopolyhedrovirus (AcNPV), which has been extensively characterized and is commercially available.
Among all the baculovirus expression related vectors, Tn7 transposition homologous recombination and gateway recombination are commonly adapted to mediate the integration. According to this classification of recombination methods, Creative BioMart offers a wide range of vectors and viral DNA corresponding to our available AcMNPV expression methods, optimized for efficient protein expression, stability and yield, so that customers can successfully process proteins in their own laboratories express.
In addition to our standard baculovirus expression vectors, we also offer customized vectors that can be tailored to meet specific customer needs. Our experienced team of scientists can assist with vector design, cloning, and protein expression optimization to ensure the highest quality and yield of recombinant proteins.