In the process of recombinant virus generation, customers may encounter many difficulties. Immature co-transfection technology, unsuitable host insect cells and the selection of corresponding media will affect the final protein expression effect. In addition, the amplification and titration process of recombinant virus may slow down the progress of whole protein expression. In order to avoid unnecessary trouble and improve the final expression efficiency, customers can consider Creative BioMart's virus generation services. From the step of transfection to the titration, Creative BioMart will always escort our customers.
In the process of baculovirus expression, the generation of recombinant virus is an important step after the construction of recombinant vector. In this step, the foreign gene is transferred into the baculovirus genome by transposition recombination or homologous recombination. The recombinant baculovirus gene then self-replicates in insect cells and guides the virus assembly. Click the links below to find out what Creative BioMart can do for you in the process of Baculovirus Generation.
Transfect the recombinant bacmid to host cell line.
Co-transfect the recombinant transfer vector and baculovirus DNA to host cell line.
Control co-transfection is also performed.
Amplify the virus stock to a titer that is high enough to be stocked or produce target protein
Perform the ALL-IN-ONE qPCR Titration
Virus titration using plaque assay
Click here to view Creative BioMart's insect cell lines and the corresponding features as well as media.
Safe storage of valuable virus stocks for future use.
After the virus generation, customers can choose to authorize us to perform subsequent protein expression services. If you choose to terminate the service, we will deliver the virus stock to you in the first time. Please know that whenever you need help, Creative BioMart will always be there.