Kinases is an enzyme that catalyzes phosphorylation process for specific substrates such as proteins, lipids, carbohydrates, etc. Phosphorylation plays an important role in metabolism, cell signal transduction, protein regulation, transportation and secretion. Many diseases and even cancer are associated with kinase dysfunction.
To facilitate the research of kinase related cancers and kinase regulatory drugs, high-quality recombinant kinases need to be provided as an important target protein. In the pharmaceutical and biotechnology industries, protein kinases are mainly produced by baculovirus expression systems, considering the factors of applicability, cost and yield. However, that kinases are inherently fragile makes its difficult to express. Therefore, helper molecules (such as chaperones) is requiored to help kinase proper folding during expression.
The essence of kinases is protein. As experts in the field of protein synthesis, Creative BioMart have been able to overcome this problem and express correctly folded and stable production of kinases. We promise to provide high quality service to our customers, and we will always be your reliable partner.
Creative BioMart's kinase expression system, through which we provide kinase expression service, is modified according to the Bac to Bac expression pathway. A package containing the ORF of 7 kinase chaperones was inserted into AcMNPV gene via the loxP site. These protein chaperones contribute to the folding of kinase enzymes, thus enhancing the stability and yield of functional proteins. Through the manipulated kinase expression approach, we can produce those previously unobtainable kinases for our customers at high levels, in soluble and functional form.
1. Gene Synthesis (optional)
2. Generating Donor Vectors that Contains Target Kinase Expression Cassettes
3. Preparation of the recombinat bacmid and recombinant baculovirus virus
4. Expression and Purification of kinase from Insect Cell
5. Delivery QC Report and Target Kinase
The expression of KRS1 was detected by SDS-PAGE with Coomassie staining. The left lane is the unmodified, and the one on the right is the modified kinase expression system. T is the total cell extract and S is the soluble protein. As shown in the figure 2, the kinase is almost invisible in the expression pathway without chaperone (the lift two). On the contrary, a high level of kinase expression was observed in the modified system.
Creative BioMart has more than 10 years of experience in protein expression and is competent to satisfy various protein expression desire. We are expert in protein expression (intracellular or secretory), signal peptide selection, insect cell selection and culture. Choosing our Kinase Expression Service, customers only need to provide the coding sequence of your target protein. We will provide customizes service from codon optimization to large-scale expression and purification. For more assistance, please contact us for help.