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Culture Amplification & Purification

Protein is the basic material of life and the basic organic matter of cells. There are many kinds of proteins with different functions. The research of protein is an indispensable part of scientific research activities. Therefore, the preparation of gram level purified protein is the premise of research activities. Large scale protein production and purification usually meet bottleneck problem in the aspect of technology and equipment.

Culture Amplification & Purification

As an expert in the field of protein expression, Creative BioMart has broken through these bottlenecks. After more than 20 years of development, we have established a complete large-scale protein expression and purification technology platform and are equipped with advanced equipment.

Creative BioMart has mature recombinant protein expression technology and protein purification technology. At present, we have a variety volume of fermentation tanks from 20L to 1000L, which can meet different production requirements. At the same time, Creative BioMart can also realize large-scale protein purification. Purification methods include affinity purification, ion exchange, molecular sieve, hydrophobic and reverse equivalence, which can effectively control the purification process.

Service Description

Large-Scale Protein Production

Culture Amplification & PurificationCreative BioMart has mature recombinant protein expression technology and protein purification technology. Our fermentation tanks support protein expression from the scale of 20L to 1000L. According to the amount of protein expression required by customers, the expression time varies from 1-3 weeks.

Note. We suggest that customers who choose this scale-up production service also choose our protein expression service, so that we can know more about your target protein and provide better expression scheme.

Large-Scale Protein Production

Culture Amplification & PurificationThe expansion of protein purification from laboratory scale to industrial scale belongs to the category of engineering. 

The key factors of large-scale protein purification are yield and cost. Creative BioMart is always trying to make the purification process as simple as possible to avoid human interference.

In the process of large-scale protein purification, the target protein, raw materials, production methods, product purity requirements and final quantity have been basically understood. Nevertheless, we will still carry out the reevaluation and make some appropriate changes to ensure the best purification result.

Service Description Timeline Deliverable
  • Enlarge culturing (according to customer demand)
  • Ni column affinity purification
  • Assay of purified protein
  • Protein delivery
  • Amplification culture
    2-3 weeks
  • Expression and purification
    1-2 weeks
  • Protein purity identification report
  • Protein product

Why Choose Creative BioMart's Culture Amplification and Protein Purification

  • We have rich experience, mature recombinant protein expression technology platform and complete equipment
  • We have the ability to conduct one-stop, complete experiments. We have the ability to complete the whole experiment from gene optimization, construction of expression plasmid and exploration of small-scale fermentation conditions to large scale fermentation
  • We provide tailored experimental scheme. Each scheme is developed under the comprehensive consideration of the physicochemical properties of protein and impurities, cost, time and other factors.

How to Order

Culture Amplification & Purification

If you are grateful for our service or have any questions, please feel free to contact us or submit online inquiry.

Please note that all products/services provided are for research use only. Not intended for any clinical use.