Even a small virus provides a huge surface, which can be manipulated in many ways. Baculovirus surface display (BSD) system one of the novel eukaryotic surface display systems developed in recent years. The target protein or polypeptide can be displayed on the surface of baculovirus by fusing with glycoprotein gp64 on baculovirus surface or binding with specific anchoring site. Due to the feature that foreign protein still maintains its original activity after the display, BSD system have become valuable tools in biotechnology and medicine.
In the BSD system, the foreign proteins are typically displayed either on the surface or the infected cell surface through fusion with viral glycoprotein GP64 (A). Generally, there are several location options for the foreign protein to be fused with—— for example the GP 64 siginal peptide (SP, B), GP 64’s transmembrane (TMD, C), GP64’s cytoplasmic tail (C-terminal domain, CTD, D), or both the TMD and CTD domains of GP64 (E).
In addation to GP64, the VP39, a major fusion partner of baculovirus’ capsid protein can also be used to conduct surface display.Based on the infusion partners (GP64 and VP39), the forgien protein can be displayed on the surface of baculovirus, baculovirus capsid, baculovirus occlusion body, infected insect cell and mammalian or other non-lepidopteran cell.
Since the advent of BSD system, it has been used to develop the low-cost insect larvae-derived recombinant subunit vaccines. Two well-known examples are the H5N1 influenza vaccine and subunit vaccines against rabbit hemorrhagic disease virus. In addition to the application on vaccines development, the BSD system has also been witnessed the protentional on:
As the newly developed surface display system, BSD several superior features that other systems do not have.
Creative BioMart provides several surface display services corresponding to 5 kinds of infusion strategies. Please click the links below to learn more about our services if you are interested in. If you have any problem, please feel free to contact us.
Use the GP64 glycoprotein as the fusion partner to display the intended protein.
Fuse the foreign protein with VP39 nucleocapsid protein (rather than GP64) and display it on the surface of baculovirus capsid
Surface display of foreign proteins using baculoviral polyhedrin as a fusion partner
Adopt the GP64 related strategy to display the protein to the host insect cells
Complete the GP64-related surface display in the non-lepidopteran cell with the help of appropriate promoters