BacMam Expression Service-Baculovirus Expression In Mammalian Cells

Baculovirus is a kind of virus which takes arthropod as a single host. Its genome has a large capacity and can accommodate the insertion of 38 kb foreign gene fragments. Studies have shown that wild baculovirus can enter mammalian cells but will not turn on the expression of its own protein, unless the mammalian promoter is inserted upstream of that gene. However, baculovirus can not replicate in mammalian cells whether it has been inserted into a recognizable promoter in mammalian cells, so it is non infectious. This feature of the baculovirus was made into a novel technology in the 1990s, which uses baculovirus to transfer foreign genes to mammals, as an improvement of the traditional baculovirus expression system, named BacMam.

Ability of baculovirus to enter mammalian cells means that transduction can be achieved by simply adding viral inoculums into cells, which have been shown to be highly efficient and dose-dependent. Moreover, the BacMam baculovirus can efficiently transduces most mammalian cells – including primary cultures, cell lines, and iPSC derived cells. Therefore, since its development, BacMam has been widely used in various fields of biotechnology and medical treatment.

The operating principle of BacMam is similar to that of the unmodified baculovirus expression system (BEVS). Baculovirus expression in mammalian cells can be achieved in a variety of pathways. However, just like the BEVS, each pathway has its own advantages and disadvantages, and cannot replace each other. As an expert in the field of biotechnology, Creative BioMart provides customers with a comprehensive construction methods of BacMam, so that customers can choose according to their needs.

What Creative BioMart Provides for Our Customers

Baculovirus expression in mammalian cells can be achieved in a variety of pathways, each pathway has its own advantages and disadvantages, and cannot replace each other. Creative BioMart provides a comprehensive construction method of BacMam. Customers who are interested in our BacMam expression service, please click the links below to learn more detail about our services. For any question or request, welcome to contact us. 

• BacMam + Bac to Bac expression approach

An approach inherits construction principle and features of the Bac-to-Bac approach

• BacMam + Gateway expression approach

The BacMam vector is constructed via Gateway recombination

• BacMam + multi gene expression approach

Support multiple gene co-expression in host mammalian cell.

Features of BacMam Expression System

Compared with other expression systems, BacMam has the following advantages

• Safe – Low biological hazard, does not replicate in mammalian cells and has no cytopathic effect
• Low toxicity – Ability to reduced overt cytotoxicity
• Efficient – Has high transduction efficiency for mammalian cell lines, primary cell lines and stem cells
• Wide host range – Can be transferred into many mammalian cell lines
• Regulatory – Gene expression can be easily regulated by changing the amount of transduction
• Rapid – Expression is much faster than generation of stable cell lines
• Delivery vectors for genes in mammalian cells.

Applications of BacMam Expression System

Including nuclear receptors, secreted proteins and membrane proteins, such as G protein coupled receptors (GPCR), drug transporters and ion channels.

• Functional characterization and protein production for structural studies
• Cell membranes production
• High content screening assay development
• Vaccine production
• Toxic proteins production