The basic principle of BEVS is to insert the target gene into the genome of baculovirus, infecting the insect host cells with recombinant baculovirus and let the cells express the protein encoded by the foreign gene.
According to this mechanism, various of improvements have been conducted to BEVS in the aspects of subclone preparation, baculovirus itself (expression element optimization and linearization), virus recombination method, recombinant virus screening method, cell culturing method, co-transfection method, protein secretion pathway, etc. The aim is to simplify the process while improving efficiency and quality. Nevertheless, each approach has its feature and cannot be replaced by others.
Creative BioMart has covered most of the existing AcMNPV BEVS approaches in the market. Click the links below to learn more information or contact us to decide a suitable approach for your protein. If you had already made your own expression plan which is beyond our list, our edge-cutting technology platform is still able to provide assistance for you. All you need to do is to contact us and put forward your requirements.
The most commonly used basic approach, and mature technology.
TOPO clone is used to improve the efficiency of recombinant donor vector preparation step.
A speedy approach. Construct expression vector in vitro within 1 hour. Save time form transforming, bacmid extraction and co-transfection.
An approach of high target gene-virus DNA recombinant rate. No need for the plaque screening and purification step.
Virus-free expression approach. Ideal for high throughput protein expression. Generate small-scale target protein within 48 hours.
Exhibiting an improved target gene-virus DNA recombinant rate.
An approach which the recombinant viruses can be detected, allowing the study of kinetics of infection and protein expression.
An expression approach with almost 100% recombinant rate
An expression approach suitable for secretory protein expression