- USA:
Product Name | 6xHis Expression and Purification Kit |
Research Area | Protein expression kit |
Application | BaculoGold |
Protein Tag | His Tag (6x) |
Unit Size | 1 Kit |
Subclone Construction Method | Restriction Enzyme ⁄ MCS |
Recombination Method | Homologous Recombination |
Promoter | polyhedrin |
Related Vector |
pAcHLT-A,B,C Baculovirus Transfer Vector Set pAcHLT-XylE Baculovirus Control Vector |
Recommend Cell Line | Sf9 |
Contents & Storage |
1. Linearized BaculoGold Baculovirus DNA (2.5 µg) 2. pAcHLT-A,B,C Baculovirus Transfer Vector Set (20 µg each) 3. pAcHLT-XylE Baculovirus Control Vector (5.0 µg) 4. Thrombin Powder ( 20 mg (1000 U)) 5. Thrombin Dilution Buffer ( 1 ml) 6. Protease Inhibitor Cocktail (lyophilized) 7. 1x Insect Cell Lysis Buffer (50 ml) 8. His Purification Resin (10 ml) 9. 6xHis Elution Buffer (40 ml) 10. 6xHis Wash Buffer (250 ml) 11. 3M Imidazole Solution 125 ml 12. Transfection Buffer A&B Set 5 ml each 13. Baculovirus Procedures & Methods Manual 1 |