Bac to Bac HBM expression system is added with a Honeybee melittin secretion signal on the donor vector to enable secreted protein expression. The protein products can either be secreted into the extracellular medium or glycosylated under the guidance of HBM. In addition, the blunt-end TOPO cloning and Tn7 site-specific transposition technology of the Bac-to-Bac System are also applied to improve the efficiency of protein expression. Consequently, customers who want to express toxic proteins and glycoproteins please consider this choice.
The Bac-to-Bac HBM TOPO secreted expression system is an innovation system based on the Bac to Bac TOPO expression system. Similarly, to establish a sound process, you may need to prepare
A Powerful, Multifunctional Vector
The pFastBac/HBM-TOPO vector supports the 5 minutes- rapid subclone construction with blunt TOPO technology while it enables secreted protein expression with its honeybee melittin (HBM) secretion signal. The vector also has a C-terminal His-Tag with a TEV cleavage signal to enable easy purification of His fusion.
One Shot Mach1 T1 Chemically Competent E. coli (BAC1204)
We supply the Mach1 T1 Chemically Competent E. coli which enables the 8-hours rapid visualization of colonies after plating.
Host Insect Cell Lines
Sf9, Sf21, High Five and Mimic Sf9 can all be used in this system. However, for transfection and identification of recombinant plaques we only recommend Sf9 or Sf21 cells. Once you have generated your baculovirus stock, High Five cell line is recommended to conduct the protein production function.
For customers who want to carry out the whole expression process, Creative BioMart provides literally everything you need in the expression kit (KTE1007) except the insect cell line. If you just want to build the construct that contains your gene of interest, please just choose the cloning kit (KTC1022). Other products like insect cell lines can all be accessed at Creative BioMart. Please click and read the instruction to decide which is best for your expression plan.