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    Bac-to-Bac

    Bac-to-Bac is one of the best development insect baculovirus expression system. The classic Bac to Bac system mainly relies on Tn7 site-specific transposition between donor plasmid and Bacmid to generate recombinant baculovirus. Its ability to efficiently prepare recombinant baculovirus and producing high concentration post-translational modified protein makes it perfectly suitable for heterologous protein expression.

    Related Products

    General Process of Bac to Bac To conduct a complete protein expression program via Bac to Bac system, you may need

    • Donor plasmid which carries the interest fragment between two Tn7 transposition site
    • Bacmid which contains Tn7 target site
    • E. coli DH10Bac which hosts Bacmid and contains a helper plasmid
    • Transfection kit or reagent
    • Insect cell line and medium
    • Protein assay products.

    As an expert in the field of protein expression, Creative Biomart not only provides one-stop protein expression services, but also provides technical guidance and product support for customers who want to conduct product expression in their own laboratories. We provide wide range of products corresponding to each expression section, so that customers are able to find all the products they need to facilitate the expression process.

    Advantages of the Products

    We offer several formats products for customer to choose flexibly. 

    • If you are looking for a simple expression complete kit.

    The Bac-to-Bac Expression Kit (KTE1001) is the basic complete expression kit which contains all vectors, insect cell line and competent cells you need.

    • If you are looking for a more efficiency expression kit

    We also provide Bac-to-Bac Expression with Gateway Technology Kit (KTE1002) which contains additional Gateway technology related vector and reagent to dramatically speed up the subclone construction step.

    • If you simply need some vectors.

    We offer several donor vectors sets (DVC1001, DVC1002, DVC1003) as well as some gateway vector products (GVC3001, GVC3002, GVC3003).

    Product Information

    Bac-to-BacIn addition to kits and vectors, Creative BioMart also provides many expression related products including insect cells, E. coli, culture media, reagent and other products. Some of these products are already included in the kit and some need to be purchased separately. You can click on the list below to learn more about the product parameters. If you have any questions, please contact us.