Bac-to-Bac TOPO is an innovated Bac-to-Bac expression system, which utilizes TOPO blunt-end clone technology to speed up donor vector construction step significantly. With the TOPO clone technology, the subclone connection can be done in 2-5 minutes, while the traditional TA clone requires 2-3 days.
The Bac-to-Bac TOPO system still relies on Tn7 site-specific transposition between donor plasmid and Bacmid to generate recombinant baculovirus. It shares the same advantages with Bac-to-Bac, while it cost shorter time on construct and screen of recombinant donor vector. To conduct a complete protein expression program via Bac to Bac TOPO system, you may need
Creative BioMart provides Bac to Bac TOPO expression kits corresponding to our comprehensive expression service. These expression kits (KTE1004, KTE1003) include either pFastBac / CT-TOPO vector or pFastBac / NT-TOPO vector to add C-His tag or N-His tag to the intended protein.
If you simply need to construct a subclone for your own expression process, our Cloning Kit (KTC1014) will be good enough to meet your requirement.
As an expert in the field of protein expression, Creative Biomart not only provides one-stop protein expression services, but also provides technical guidance and product support for customers who want to conduct product expression in their own laboratories. We provide wide range of products corresponding to each expression section, so that customers are able to find all the products they need to facilitate the expression process.