BacPAK expression system adopts the rescue strategy to solve the problem. The viral is pre-linearized at three Bsu36I restriction enzyme recognition sites. A part of the essential ORF1629 is cut off by the enzyme, thus the linear viral DNA can neither self-linkage nor survive in a non-recombined form.
Creative BioMart provides various types of Expression Kits, Titration kits, Transfer vectors, Liner viral DNA, Mediums and Insect cell lines for customers who are interested in our BacPAK related products.
The Complete Kits and Target Infused vector for High Yield Recombinant Protein Expression
The classic complete expression kit (KTE1010) contains almost everything you need for each reaction. The kit offers a BacPAK6 pre-linearized viral DNA, three kinds of transfer vector (pBacBAK8, pBacBAK9) as well as a control vector. The kit also packs the recommended insect cell line inside.
| Product Name | Function |
| pBacPAK8-C term 6xHN | C terminal 6xHN |
| pBacPAK8-N term 6xHN | N terminal 6xHN |
| pBacPAK-N term 6xHN-GUS | Positive control |
If you want to conduct tag infusion while expressing the recombinant protein, please purchase in-Fusion Ready BacPAK Vectors instead of using the conventional pBacPAK transfer vectors in the complete kit. Creative BioMart offers pBacPAK8-N/Cterm 6xHN vectors (TVC2114/ TVC2115) transfer vectors which contain coding sequence of 6xHN (polyhistidine) is attached at the C- or N-terminal of the transfer vector. The recombinant protein product with 6xHN tag can be easily purified by using TALON resin.
The both kits exhibit the feature of:
If you wish, you can use pBacPAK8-GUS (pBacPAK-N term 6xHN-GUS Vector for infusion expression) as a positive control for the co-transfection step. This transfer vector has the E. coli beta-glucuronidase (GUS) gene cloned downstream of its polyhedrin promoter. Recombination of pBacPAK8-GUS with the BacPAK6 DNA digest generates recombinant viruses that express beta-glucuronidase. Expression of GUS can be detected by generation of a blue color from the chromogenic GUS substrate X-GLUC.
Please note: We provide flexible product selection. You can purchase the transfer vector in the form of single or vector set according to your needs. If you have other needs, please feel free to contact us or turn to the online inquiry.
As an expert in the field of protein expression, Creative Biomart not only provides one-stop protein expression services, but also provides technical guidance and product support for customers who want to conduct product expression in their own laboratories. We provide wide range of products corresponding to each expression section, so that customers are able to find all the products they need to facilitate the expression process.