Rescue strategy is utilized in FlashBAC expression system to ensure the high homologous recombination rate. The essential gene ORF 1629 is partly delated. Consequently, the non-recombinant virus gene backbone cannot self-replicate when transfected into insect cell. Polh coding sequence is replaced with a bacterial artificial chromosome (BAC) at the polh locus, allowing the virus genome to be maintained in bacterial cells as a bacmid.
Benefit from the brilliant rescue strategy and the baculovirus virus backbone manipulation, the recombinant rate between transfer vector and virus backbone can reach 100%.
For customers who are interested in our FlashBAC related products, Creative BioMart provides various types of Expression Kits, Transfection Kits & related reagents, Titration kits, Transfer vectors, Liner DNA, Mediums and Insect cell lines.
Various of transfer vectors and modified Liner viral DNA
This system consists of an AcMNPV genome as well as a transfer vector. Creative BioMart offers 5 subsystems (FlashBAC, FlashBAC GOLD, FlashBAC ULTRA, FlashBAC PRIMER) related product and it is the different viral DNA that distinguishes one subsystem form each other.
| Name | Modification | Feature |
| FlashBAC | Backbone virus DNA chiA delectation | A. Prevent production of virus chitinase. B. Improved membrane and secreted protein production. |
| FlashBAC GOLD | Backbone virus DNA chiA and v-cath delectation | A. Prevent production of virus chitinase and cathepsin. B. Improved membrane and secreted protein production. C. Prevent susceptible protein degradation. |
| FlashBAC ULTRA | Backbone virus DNA chiA, v-cath and p10/p26/p74 delectation | A. Prevent production of virus chitinase and cathepsin. B. Improved membrane and secreted protein production. C. Prevent susceptible protein degradation. D. Extend protein production times in T. ni Hi5 cells. E. Reduces the metabolic burden on the cell |
| FlashBAC PRIME | No gene deletions. |
The transfer vector corresponding to these subsystems are universal. Creative BioMart offers competent transfer vectors which facilities His x 6 tag infusion, HBM signal sequence guided secretion and mammalian cell expression. These vectors can also be used to participate any baculovirus expression system that utilizes homologous recombination at the polyhedrin locus in insect cells (or mammalian cells). In addition to some of the vectors recommended by us, you can also choose other vectors according to your needs.
Expression and purification kit options
We provide a variety of kit combination options. customers can choose according to their needs. We have basic protein expression kit, complete protein expression and virus titration kit, virus generation and titration kit for different subsystems, and basic titration kit.
As an expert in the field of protein expression, Creative Biomart not only provides one-stop protein expression services, but also provides technical guidance and product support for customers who want to conduct product expression in their own laboratories. We provide wide range of products corresponding to each expression section, so that customers are able to find all the products they need to facilitate the expression process.