| Product Name | Bac-to-Bac HBM TOPO Cloning Kit |
| Research Area | Vector construction kit and reagent |
| Application | Bac-to-Bac HBM |
| Protein Tag | HBM (Honeybee Melittin),His Tag (6x) |
| Kit Function | Donor vector construction kit |
| Unit Size | 20 reactions |
| Subclone Construction Method | Blunt TOPO |
| Recombinant Vector Screening | lacZ gene |
| Promoter | Polyhedrin |
| Related Vector | pFastBac/HBM-TOPO |
| Recommend Cell Line | Sf9, Mimic Sf9, Sf21, and High Five cells in Sf-900 II & Sf-900™ III media |
| Contents & Storage |
Bac-to-Bac HBM TOPO Cloning Kit contains: 1. Vector kit for 20 topo cloning reactions 2. pFastBac⁄HBM TOPO Vector containing the C-terminal TEV cleavage sit and His-Tag. 3. pFastBac⁄CT-Gus Control Plasmid (Control expression vector) 4. Other reagents supplied: 10x PCR, dNTP Mix, Salt Solution, Sterile Water, Control PCR template, Polyhedrin Forward Primer, SV40 pA Reverse Primer 5. Kit containing competent cells (20 reactions): 6. One Shot Mach1-T1R Chemically Competent E. coli Vector kit: store at -20°C One Shot Mach1-T1R Chemically Competent E. coli: store at -80°C |