| Product Name | Bac-to-Bac HBM TOPO Secreted Expression System |
| Research Area | Protein expression kit |
| Application | Bac-to-Bac HBM |
| Protein Tag | His Tag (6x) |
| Unit Size | 20 reactions |
| Subclone Construction Method | Blunt TOPO |
| Recombinant Vector Screening | lacZ gene |
| Recombination Method | Tn7 transposition |
| Promoter | Polyhedrin |
| Related Vector | pFastBac/HBM-TOPO Vector |
| Recommend Cell Line | Sf9, Sf21, and High Five |
| Contents & Storage |
1. Vector kit for 20 TOPO cloning reactions, store at -20°C –pFastBac/HBM-TOPO Vector containing C-terminal TEV cleavage site and His-tag –pFastBac 1-Gus control plasmid (control expression vector) –Other kit reagents: 10X PCR, dNTP mix, Salt solution, sterile water, control PCR template, polyhedrin forward primer, SV40 pA reverse primer 2. Mach1-T1R Chemically Competent E. coli ( Cat. NO BAC1204) for 20 reactions (20 x 50 µL), store at -80°C 3. DH10Bac Competent E. coli ( Cat. NO BAC1202) for 20 reactions (4 kits with 5 x 0.1 mL each), store at -80°C 4. Sf Transfection Reagent (1 mL) ( Cat. NO KTR1029) , store at 4°C, do not freeze |