| Product Name | Bac-to-Bac TOPO Cloning Kit, C-His |
| Research Area | Vector construction kit and reagent |
| Application | Bac-to-Bac TOPO |
| Protein Tag | His Tag (6x) |
| Kit Function | Donor vector construction kit |
| Unit Size | 20 reactions |
| Subclone Construction Method | Blunt TOPO |
| Antibiotic Resistance Bacterial | Ampicillin (AmpR), Gentamicin (GmR) |
| Recombinant Vector Screening | lacZ gene |
| Promoter | Polyhedrin |
| Related Vector | pFastBac⁄CT-TOPO |
| Recommend Cell Line | Sf9, Sf21, and High Five |
| Contents & Storage |
1. Vector kit for 20 topo cloning reactions: pFastBac⁄CT-TOPO Vector containing the C-terminal TEV cleavage sit and His-Tag. pFastBac⁄CT-Gus Control Plasmid (Control expression vector) Other reagents supplied: 10x PCR, dNTP Mix, Salt Solution, Sterile Water, Control PCR template, Polyhedrin Forward Primer, SV40 pA Reverse Primer 2. Kit containing competent cells (20 reactions): One Shot Mach1-T1R Chemically Competent E. coli Vector kit: store at -20°C One Shot Mach1-T1R Chemically Competent E. coli: store at -80°C |