| Product Name | BacPAK Baculovirus Expression System |
| Research Area | Protein expression kit |
| Application | BacPAK |
| Protein Tag | N/A |
| Unit Size | each |
| Subclone Construction Method | Restriction Enzyme ⁄ MCS |
| Recombinant Vector Screening | PCR amplification with Bac1 and Bac2 Primers |
| Recombination Method | Homologous Recombination |
| Promoter | polyhedrin |
| Related Vector | pBacPAK8, pBacPAK9, pBacPAK8-GUS |
| Recommend Cell Line | Sf21 |
| Contents & Storage |
Store the following item at –180°C (liquid nitrogen) immediately upon receipt. 1. 2 x 10^6 IPLB-Sf21 Insect Host Cells in TNM-FH/10% FBS/ 10% DMSO Store the following items at 4°C; do not freeze. 2. 25 µl BacPAK6 Viral DNA (Bsu36 I digest) 3. 25 µl Bacfectin For long-term storage, store the following reagents at –20°C. For storage less than 6 months, store at 4 °C. 4. 15 µg pBacPAK8 Transfer Vector (500 ng/µl) 5. 15 µg pBacPAK9 Transfer Vector (500 ng/µl) 6. 2.5 µg pBacPAK8-GUS Vector (100 ng/µl) 7. 20 µl Bac1 Primer (20 µM) 8. 20 µl Bac2 Primer (20 µM) |