| Product Name | DH10B T1 Phage-ResistantvCells |
| Research Area | Bacteria Competent Cells |
| Application | Bac-to-Bac |
| Function | Facilities the construction of recombinant bacmid |
| Bacterial Strain | DH10Bac |
| Unit Size | 20 reactions |
| Screening Method | Blue/White Screening |
| Cloning Methylated DNA | yes |
| Competent Cell Type | Chemically competent |
| F'episome (to make ssDNA) | Lacks F' Episome |
| High-throughput Compatibility | Not High-throughput Compatible |
| Preparing Unmethylated DNA | Not Suitable for preparing unmethylated DNA |
| Propagating ccdB Vectors | Not for ccdB vector propagation |
| Reduces Recombination | yes |
| T1 Phage - Resistant (tonA) | yes |
| Transformation Efficiency | > 1x10^9 |
| Transformation Efficiency Level | High Efficiency (> 10^9 cfu⁄µg) |
| Improves Plasmid Quality | yes |
| Format | one shot |
| Species | E. coli |
| Contents & Storage | Store in ultra-cold freezer (-68 to -85°C). |
| Feature |
1. tonA genotype to confer resistance to T1 and T5 phage 2. Elimination of mcrA, mcrBC, mrr, and hsdRMS restriction systems to allow construction of more representative genomic libraries (1,2) 3. The endA1 mutation to increase plasmid yield and quantity 4. ZΔM15 for blue/white screening of recombinant clones |