| Product Name | DH10Bac Competent Cells |
| Research Area | Bacteria Competent Cells |
| Application |
Bac-to-Bac Bac-to-Bac TOPO |
| Function | Facilities the construction of recombinant bacmid |
| Bacterial Strain | DH10Bac |
| Unit Size | 5 x 100 µL |
| Screening Method | Blue/White Screening |
| Cloning Methylated DNA | NO |
| Competent Cell Type | Chemically competent |
| F'episome (to make ssDNA) | Lacks F' Episome |
| High-throughput Compatibility | Not High-throughput Compatible |
| Preparing Unmethylated DNA | Not Suitable for preparing unmethylated DNA |
| Propagating ccdB Vectors | Not for ccdB vector propagation |
| Reduces Recombination | no |
| T1 Phage - Resistant (tonA) | no |
| Transformation Efficiency | > 1x10^8 |
| Transformation Efficiency Level | Medium Efficiency (10^8-10^9 cfu⁄µg) |
| Improves Plasmid Quality | no |
| Cloning Unstable DNA | Not Suitable for cloning unstable DNA |
| Shipping Condition | Dry Ice |
| Format | Tube(s) |
| Species | E. coli |
| Contents & Storage |
1. DH10Bac Chemically Competent Cells: 5 vials, 100 µl each (total of 500 µl) 2. pUC19 DNA (0.01 µg/ml): 1 vial, 100 µl 3. SOC Medium: 2 bottles, 6 ml each Store Competent Cells at -80°C. Store pUC19 DNA at -20°C. Store SOC Medium at 4°C or at room temperature. |
| Feature |
Features of MAX Efficiency DH10Bac Competent Cells include: 1. Consistent yields of >1 × 10^8 transformants/µg 2. Tolerance of small amounts of undiluted ligation reactions 3. Support of efficient blue/white colony screening (Φ80lacZΔM15 marker) |