• USA:
    1-631-559-9269 1-516-512-3133

DH5α Competent Cells

  • SPECIFICATION
  • Cat No. BAC1203
Product Name DH5α Competent Cells
Research Area Bacteria Competent Cells
Application Gateway +Bac-to-Bac
Function Facilities the construction of recombinant bacmid
Bacterial Strain DH5α
Unit Size 200 µL
Screening Method Blue/White Screening
Cloning Methylated DNA NO
Competent Cell Type Chemically competent
F'episome (to make ssDNA) Lacks F' Episome
High-throughput Compatibility Not High-throughput Compatible
Preparing Unmethylated DNA Not Suitable for preparing unmethylated DNA
Propagating ccdB Vectors Not for ccdB vector propagation
Reduces Recombination yes
T1 Phage - Resistant (tonA) no
Transformation Efficiency > 1x10^8
Transformation Efficiency Level Medium Efficiency (10^8-10^9 cfu⁄µg)
Improves Plasmid Quality yes
Cloning Unstable DNA Not Suitable for cloning unstable DNA
Shipping Condition Dry Ice
Format Tube(s)
Species E. coli
Contents & Storage 1.DH5α Competent Cells: 5 vials, 200 µl each (total of 1 ml)
2. pUC19 DNA (10 pg/ul): 1 vial, 50 µl
3. S.O.C. medium: 2 bottles, 6 ml each
Store Competent Cells at -80°C. Store pUC19 DNA at -20°C. Store SOC Medium at 4C or at Room Temperature.
Feature 1. Supports blue/white screening by α-complementation on plates containing X-Gal or Bluo-Gal
2. Maintains plasmid DNA with minimum recombination events
3. Delivers high-yield plasmid preparations for downstream applications
4. M13mp cloning vectors may be propagated on a lawn of DH5α-FT, DH5αF', DH5αF'IQ, JM101, or JM107 for plaque formation
Please note that all products/services provided are for research use only. Not intended for any clinical use.
Online Inquiry

We use cookies to understand how you use our site and to improve the overall user experience. This includes personalizing content and advertising. Read our Privacy Policy

Accept Cookies
x