| Product Name | DH5α Competent Cells |
| Research Area | Bacteria Competent Cells |
| Application | Gateway +Bac-to-Bac |
| Function | Facilities the construction of recombinant bacmid |
| Bacterial Strain | DH5α |
| Unit Size | 200 µL |
| Screening Method | Blue/White Screening |
| Cloning Methylated DNA | NO |
| Competent Cell Type | Chemically competent |
| F'episome (to make ssDNA) | Lacks F' Episome |
| High-throughput Compatibility | Not High-throughput Compatible |
| Preparing Unmethylated DNA | Not Suitable for preparing unmethylated DNA |
| Propagating ccdB Vectors | Not for ccdB vector propagation |
| Reduces Recombination | yes |
| T1 Phage - Resistant (tonA) | no |
| Transformation Efficiency | > 1x10^8 |
| Transformation Efficiency Level | Medium Efficiency (10^8-10^9 cfu⁄µg) |
| Improves Plasmid Quality | yes |
| Cloning Unstable DNA | Not Suitable for cloning unstable DNA |
| Shipping Condition | Dry Ice |
| Format | Tube(s) |
| Species | E. coli |
| Contents & Storage |
1.DH5α Competent Cells: 5 vials, 200 µl each (total of 1 ml) 2. pUC19 DNA (10 pg/ul): 1 vial, 50 µl 3. S.O.C. medium: 2 bottles, 6 ml each Store Competent Cells at -80°C. Store pUC19 DNA at -20°C. Store SOC Medium at 4C or at Room Temperature. |
| Feature |
1. Supports blue/white screening by α-complementation on plates containing X-Gal or Bluo-Gal 2. Maintains plasmid DNA with minimum recombination events 3. Delivers high-yield plasmid preparations for downstream applications 4. M13mp cloning vectors may be propagated on a lawn of DH5α-FT, DH5αF', DH5αF'IQ, JM101, or JM107 for plaque formation |