| Product Name | DH5α-T1R Competent Cells |
| Research Area | Bacteria Competent Cells |
| Application | Bac-to-Bac |
| Function | Facilities the construction of recombinant bacmid |
| Bacterial Strain | DH5α |
| Unit Size | 20 reactions |
| Screening Method | Blue/White Screening |
| Cloning Methylated DNA | no |
| Competent Cell Type | Chemically competent |
| F'episome (to make ssDNA) | Lacks F' Episome |
| High-throughput Compatibility | Not High-throughput Compatible |
| Preparing Unmethylated DNA | Not Suitable for preparing unmethylated DNA |
| Propagating ccdB Vectors | Not for ccdB vector propagation |
| Reduces Recombination | yes |
| T1 Phage - Resistant (tonA) | yes |
| Transformation Efficiency | > 1x10^9 |
| Transformation Efficiency Level | High Efficiency (> 10^9 cfu⁄µg) |
| Improves Plasmid Quality | yes |
| Format | one shot |
| Species | E. coli |
| Contents & Storage |
1. DH5α-T1R Competent Cells: 20 vials, 50 µl each ( total of 1 ml) 2. pUC19 DNA (10 pg/ul): 1 vial, 50 µl 3. S.O.C. medium: 1 bottle, 6 ml Store Competent Cells at -80°C. Store pUC19 DNA at -20°C. Store SOC Medium at 4°C or at room temperature |
| Feature |
1. Cleaner preparations of DNA and better results in downstream applications due to the elimination of nonspecific digestion by Endonuclease I (endA1) 2. Reduced occurrence of unwanted recombination in cloned DNA (recA1) 3. Efficient transformation of unmethylated DNA from PCR applications (hsdR) 4. Blue/white color screening of recombinant clones carrying the alpha fragment of β-galactosidase (lacZΔM15) |