| Product Name | Mach1 T1 Chemically Competent E. coli |
| Research Area | Bacteria Competent Cells |
| Application | Bac-to-Bac TOPO |
| Function | Facilities the construction of recombinant bacmid |
| Bacterial Strain | Mach1 |
| Unit Size | 20 x 50 µL |
| Screening Method | Blue/White Screening |
| Cloning Methylated DNA | NO |
| Competent Cell Type | Chemically competent |
| F'episome (to make ssDNA) | Lacks F' Episome |
| High-throughput Compatibility | Not High-throughput Compatible |
| Preparing Unmethylated DNA | Not Suitable for preparing unmethylated DNA |
| Propagating ccdB Vectors | Not for ccdB vector propagation |
| Reduces Recombination | yes |
| T1 Phage - Resistant (tonA) | yes |
| Transformation Efficiency | > 1x10^9 |
| Transformation Efficiency Level | High Efficiency (> 10^9 cfu⁄µg) |
| Improves Plasmid Quality | yes |
| Cloning Unstable DNA | Not Suitable for cloning unstable DNA |
| Shipping Condition | Dry Ice |
| Quantity | 20 x 50μL |
| Format | one shot |
| Species | E. coli |
| Contents & Storage |
1. Mach1 Chemically Competent E. coli: 21 vials, 50 µl each 2. pUC19 DNA (10 pg/µl): 1 vial, 50 µl 3. S.O.C. medium: 1 bottle, 6 ml Store Competent Cells at -80°C. Store pUC19 DNA at -20°C. Store SOC Medium at 4°C or at room temperature. |
| Feature |
1. Minimizes nonspecific recombination in cloned DNA (recA) 2. Offers resistance to T1 and T5 phage (tonA) 3. Yields cleaner preparations of DNA and better results in downstream applications due to the elimination of nonspecific digestion by Endonuclease I (endA1) 4. Provides efficient transformation of unmethylated DNA from PCR amplifications (hsdR) 5. Allows blue/white screening of recombinant clones (lacZΔM15) |