| Product Name | pFastBacHT C |
| Research Area | Donor Vector |
| Application | Bac-to-Bac |
| Protein Tag | N-His |
| Vector Size | 4858 bp |
| Antibiotic Resistance | Ampicillin (AmpR) |
| Cloning Sites | Restriction Enzyme ⁄ MCS |
| Colony screening | lacZα |
| Promoter | Polyhedrin |
| Recombination Site | Tn7L/Tn7R |
| Recommend Cell Line | Sf9, Sf21 |
| Contents & Storage | -20 ℃ |
| Feature |
The pFastBac HT vector series is supplied with multiple cloning sites in three reading frames (A, B, and C), facilitating in-frame fusion of the gene of interest with an N-terminal polyhistidine tag. Key features include a strong baculovirus polyhedrin promoter for high-level expression in insect cells, an N-terminal polyhistidine tag for affinity purification, and a site-specific protease cleavage sequence for optional removal of the affinity tag following purification. The availability of three reading frames simplifies cloning and expression optimization. |
