- USA:
Product Name | TOP10 Chemically Competent E. coli-10 reaction |
Research Area | Bacteria Competent Cells |
Application | Bac-to-Bac |
Function | Facilities the construction of recombinant bacmid |
Bacterial Strain | TOP10 |
Unit Size | 10 reactions |
Screening Method | Blue/White Screening |
Cloning Methylated DNA | yes |
Competent Cell Type | Chemically competent |
F'episome (to make ssDNA) | Lacks F' Episome |
High-throughput Compatibility | Not High-throughput Compatible |
Preparing Unmethylated DNA | Not Suitable for preparing unmethylated DNA |
Propagating ccdB Vectors | Not for ccdB vector propagation |
Reduces Recombination | yes |
T1 Phage - Resistant (tonA) | no |
Transformation Efficiency | > 1x10^9 |
Transformation Efficiency Level | High Efficiency (> 10^9 cfu⁄µg) |
Improves Plasmid Quality | yes |
Shipping Condition | Dry Ice |
Quantity | 10 reactions |
Format | one shot |
Species | E. coli |
Contents & Storage |
1. One Shot TOP10 Chemically Competent E. coli: 11 vials, 50 µl each 2. pUC19 DNA (10 pg/µl): 1 vial, 50 µl 3.S.O.C. medium: 1 bottle, 6 ml Store Competent Cells at -80°C. Store pUC19 DNA at -20°C. Store SOC Medium at 4°C or at room temperature. |
Feature |
1. hsdR for efficient transformation of unmethylated DNA from PCR amplifications 2. mcrA for efficient transformation of methylated DNA from genomic preparations 3. lacZΔM15 for blue/white color screening of recombinant clones 4. endA1 for cleaner DNA preparations and better results in downstream applications due to elimination of nonspecific digestion by Endonuclease I 5. recA1 for reduced occurrence of nonspecific recombination in cloned DNA |