| Product Name | TOP10 Chemically Competent E. coli-20 reaction |
| Research Area | Bacteria Competent Cells |
| Application | Bac-to-Bac |
| Function | Facilities the construction of recombinant bacmid |
| Bacterial Strain | TOP10 |
| Unit Size | 20 reactions |
| Screening Method | Blue/White Screening |
| Cloning Methylated DNA | yes |
| Competent Cell Type | Chemically competent |
| F'episome (to make ssDNA) | Lacks F' Episome |
| High-throughput Compatibility | Not High-throughput Compatible |
| Preparing Unmethylated DNA | Not Suitable for preparing unmethylated DNA |
| Propagating ccdB Vectors | Not for ccdB vector propagation |
| Reduces Recombination | yes |
| T1 Phage - Resistant (tonA) | no |
| Transformation Efficiency | > 1x10^9 |
| Transformation Efficiency Level | High Efficiency (> 10^9 cfu⁄µg) |
| Improves Plasmid Quality | yes |
| Shipping Condition | Dry Ice |
| Quantity | 20 reactions |
| Format | one shot |
| Species | E. coli |
| Contents & Storage |
1. One Shot TOP10 Chemically Competent E. coli: 21 vials, 50 µl each 2. pUC19 DNA (10 pg/µl): 1 vial, 50 µl 3.S.O.C. medium: 1 bottle, 6 ml Store Competent Cells at -80°C. Store pUC19 DNA at -20°C. Store SOC Medium at 4°C or at room temperature. |
| Feature |
1. hsdR for efficient transformation of unmethylated DNA from PCR amplifications 2. mcrA for efficient transformation of methylated DNA from genomic preparations 3. lacZΔM15 for blue/white color screening of recombinant clones 4. endA1 for cleaner DNA preparations and better results in downstream applications due to elimination of nonspecific digestion by Endonuclease I 5. recA1 for reduced occurrence of nonspecific recombination in cloned DNA |