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TOP10 Chemically Competent E. coli-40 reaction

  • SPECIFICATION
  • Cat No. BAC1207
Product Name TOP10 Chemically Competent E. coli-40 reaction
Research Area Bacteria Competent Cells
Application Bac-to-Bac
Function Facilities the construction of recombinant bacmid
Bacterial Strain TOP10
Unit Size 40 reactions
Screening Method Blue/White Screening
Cloning Methylated DNA yes
Competent Cell Type Chemically competent
F'episome (to make ssDNA) Lacks F' Episome
High-throughput Compatibility Not High-throughput Compatible
Preparing Unmethylated DNA Not Suitable for preparing unmethylated DNA
Propagating ccdB Vectors Not for ccdB vector propagation
Reduces Recombination yes
T1 Phage - Resistant (tonA) no
Transformation Efficiency > 1x10^9
Transformation Efficiency Level High Efficiency (> 10^9 cfu⁄µg)
Improves Plasmid Quality yes
Shipping Condition Dry Ice
Quantity 40 reactions
Format one shot
Species E. coli
Contents & Storage 1. One Shot TOP10 Chemically Competent E. coli: 42 vials, 50 µl each
2. pUC19 DNA (10 pg/µl): 1 vial, 50 µl
3.S.O.C. medium: 1 bottle, 6 ml
Store Competent Cells at -80°C. Store pUC19 DNA at -20°C. Store SOC Medium at 4°C or at room temperature.
Feature 1. hsdR for efficient transformation of unmethylated DNA from PCR amplifications
2. mcrA for efficient transformation of methylated DNA from genomic preparations
3. lacZΔM15 for blue/white color screening of recombinant clones
4. endA1 for cleaner DNA preparations and better results in downstream applications due to elimination of nonspecific digestion by Endonuclease I
5. recA1 for reduced occurrence of nonspecific recombination in cloned DNA
Please note that all products/services provided are for research use only. Not intended for any clinical use.
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