Bac-to-Bac HBM Secreted Protein Expression Service

Baculovirus expression system can express a variety of recombinant proteins, which can be divided into secretory and non-secretory proteins according to the presence or absence of signal peptide. In order to further improve the expression efficiency and yield of secretory protein, Creative BioMart provides Bac to Bac HBM secret protein expression service. The expression system is not only suitable for high-level expression of secretory proteins, but also can be used to mediate the secretory expression of toxic proteins to reduce the metabolic pressure of insect cells.

Creative BioMart has more than 10 years of experience in protein expression and is competent to satisfy various protein expression desire. We are expert in protein expression (intracellular or secretory), signal peptide selection, insect cell selection and culture. Choosing our comprehensive service, customers only need to provide the coding sequence of your target protein. We will provide customizes service from codon optimization to large-scale expression and purification.

Technology Description

The Bac-to-Bac HBM TOPO secreted expression system is an innovation system based on the Bac to Bac TOPO expression system. Similarly, it consists of

• A donor vector
• Contains a N-terminal Honey Bee Mellitin (HBM) secretion signal coding sequence to facilitate the recombinant protein secretion into the extracellular medium.
• Also contains a C-terminal polyhistidine tag that allows easy purification of the secreted protein.
• E. coli host strain which contains a bacmid.

The gene fragment of interest can be cloned to donor vector via the blunt-end TOPO cloning technology in only 5 mins. The gene fragment is then inserted to bacmid from donor vector through Tn7 site-specific transposition. After bacmid infecting insect cell lines, the recombinant protein could be collected, directly in the culture medium.

Feature of Bac-to-Bac TOPO Protein Expression Service

The Bac-to-Bac HBM TOPO secreted expression system shows following advantages over the traditional method

• Construct the donor vector with highly efficient one-step TOPO clone technology, thus allowing the use of proofreading polymerases in the PCR amplification step.
• The ultimate protein product is secreted out of the cell automatically. Thus, save the time from protein separation and purification.
• Only requires less than 2 weeks to identify and purify a recombinant baculovirus.
• Reduces the need for multiple rounds of plaque purification
• Permits rapid and simultaneous isolation of multiple recombinant baculoviruses
• Allows secreted expression of protein variants for structure/function studies.

Service Details of Bac-to-Bac TOPO System

Here is the service process, which is very flexible. Customers can choose to start from or stop at any step they want. For more technical information or enquiry, please feel free to contact us.