Features of Different Recombinant Protein Expression Systems

Baculovirus Expression Vector System (BEVS) is also known as Insect Cell Expression System. Protein expression in this system is based on the baculovirus-infected insect cells that have the capability to form properly folded protein and undertake post translation modification.

Compared with other eukaryotic expression systems, BEVS offers advantages including:

Features of Different Recombinant Protein Expression Systems

High foreign DNA capacity
High recombinant protein yield
Support heteropoly protein expression
Support post-translation modification and protein folding
Require simple cultivation conditions
Biosecurity

On top of the basic protein expression function, BEVS had been utilized in research, and medical, agriculture field for various purpose. The newly developed Baculovirus Surface Display Technique and BacMam Gene Delivery Technology make BEVS begin to show its advantages in the field of biomedical including protein interaction research, antibody and vaccine development, gene therapy, drug targeted delivery, etc.

Comparison of Different Recombinant Protein Expression Systems

Expression system	Application	Advantage	Limitation
Mammalian	Protein structure analysis Protein function analysis Antibody production Expression of complex proteins Protein interaction Virus generation	The highest level of protein processing Protein can be produced by transient or stable expression Reliable optimized instantaneous system can realize fast and ultra-high protein production	High yield can be achieved only in suspension culture Rigorous training conditions
Baculovirus (insect cell)	Protein structure analysis Protein function analysis Intracellular protein expression Protein complex expression Virus generation	Similar to mammalian protein processing Post translation modification can be completed It can be used for static or suspension culture It is harmless to non-arthropods The expression system has strong plasticity and large capacity	The culture condition is more severe than that of prokaryotic system It is time-consuming to generate recombinant baculovirus vector
Yeast	Protein structure analysis Antibody production Protein function analysis Protein interaction analysis	Eukaryotic protein processing It can realize large-scale fermentation Simple medium requirements	Fermentation is needed to get higher yield Training conditions need to be optimized
Bacterial	Protein structure analysis Antibody production Protein function analysis Protein interaction analysis	Scalable scale low cost The cultivation conditions are simple	Affected by protein solubility Protein specific optimization may be required It is difficult to express some mammalian proteins
Algal	Study of photosynthesis, plant biology, lipid metabolism genetic engineering Biofuel production	Gene modification and expression system for photosynthetic algae It is suitable for the production of biofuels, nutritional products and special chemicals Optimization system can achieve stable screening and expression	New technology Compared with other host platforms, the degree of development is low
Cell-free	Toxic protein Integration of unnatural markers or amino acids Protein function analysis Protein interaction analysis Screening of translation inhibitors	Open system, able to add non-natural components It can realize fast expression Easy to operate	The cost of scaling up to the milligram scale may be higher

Creative BioMart has developed a comprehensive BEVS platform, on which our customers can not only express their protein with a variety of pathways and vector viruses, but also benefit from new technology services like protein surface display technology and BacMam. Meanwhile, Creative BioMart provides almost everything you need so that customers can successfully conduct the protein expression in their own Lab.

Please note that all products/services provided are for research use only. Not intended for any clinical use.