Protein Qualitative Assay Service-De novo Protein Sequencing

As the follow up section of protein expression, it is essential to conduct protein qualitative assay to make sure the produced protein is consistent with the desired one.

To evaluate whether a recombinant protein is correctly expressed, it is necessary to check not only whether the amino acids form the peptide chain as expected, but also the peptide chain folding and post-translational modification. Baculovirus expression system has excellent ability of protein folding and post-translational modification. The protein folding processed by the system is always consistent with wild-type proteins perfectly. However, baculovirus expression systems are not always perfect for post-translational modifications, such as some glycosylation modifications.

To address the problem, Creative BioMart provides De novo protein sequencing service, through which research can check whether the pre-set post-translational modification has been performed correctly while sequencing the peptide chain.

TECHNOLOGY DESCRIPTION

The traditional protein sequence analysis technology typically relies on the protein database. The mass spectrometry (MS) results were matched with the existing protein data in the database to determine the primary sequence of the protein. The limitation is obvious — if the protein is not included in the database, then the protein cannot be sequenced.

Different from traditional methods, de novo does not need to rely on any database to analyze the results. The peptide sequence can be directly deduced from the experimental MS / MS spectrum by some calculation methods. It can be used to assay the non-sequenced biological proteins, antibodies, post-translational modified (PTM) peptides and endogenous peptides.

SERVICE CONTENT

• Sequencing the recombinant that does not exist in the protein library.

It can be used to detect non sequenced biological proteins and unknown proteins, and to reverse engineer enzymes and other protein molecules.

• Identify post-translational modifications and mutations, and accurately determine the sequence of proteins.
• In combination with matrix assisted laser desorption ionization peptide fragment fragmentation technology, protein identification can be carried out on colloidal samples stained with test or silver

TECHNOLOGY FEATURE

• Combined with ESI peptide fragmentation technology, complex samples such as SDS-PAGE samples can be identified.
• High sensitivity, sequencing process requires only a small number of samples.
• High throughput, a variety of proteins can be identified at one time with high automation, strong separation ability, fast analysis speed and good separation effect.
• It can be used to analyze protein bands, immunoprecipitation eluent, tissue extract, whole cell lysate and subcellular separation components.

HOW TO ORDER

Creative BioMart has been committed to protein expression, purification and analysis for many years, and has established an advanced technology platform. Our professional technical team can provide appropriate protein sequence services according to the protein provided by customers.