Creative BioMart provides various vectors and cell lines as competent cells contents. Vector with different MCS, promoter and antibiotic resistance, E. coli strains and insect cell line with different elements and features are all listed below. Customers can check the corresponding descriptions and decide their intended ones.
Four types of acceptor vectors are currently available at Creative BioMart. The pACEBac 1and 2 harbors one MCS flanked by Polh or/ and p10 promoter while the pKL and pFL contains 2. The homing endonuclease site I–Ceul and BstXI site form a multiplication mode M, allowing integration of multiple gene cassettes. SV40 and HSVtk are terminators. The whole expression sequence is encompassed with Tn7 which can induce transposition. There is also a LoxP site on the vector, facilitating the Cre-Lox recombination.
Five donor vectors are currently available at Creative BioMart. The over-all design of donor vectors is similar to that of acceptor vector, but there is no Tn7 site on donor vector, meaning that the donor vector need to fusion with acceptor vector to complete the approach. In the case of donor vector, the HE site is consists of PI-Scel and BstXI. Different vector show resistance to different antibiotics, but all vectors contain a LoxP incomplete inverted repeat to create acceptor-donor or donor-donor fusions.
Two baculoviral genes, v-cath and chiA, have been knocked out to eliminate V-cath activity and to enable chitin-affinity chromatography for purification without interference from the chiA gene product. The quality of proteins produced is significantly improved.
The EMBacY backbone contains a constitutively expressing YFP expression cassette which allows for easy monitoring of viral titers via fluorescence without plaque assays.
The MultiBac backbone is identical to EMBacY except missing the YFP cassette.
| Insect Cell | Advantage | Use |
| Sf9 (Spodoptera frugiperda) | Well-characterized host cell, good for plaquing | All general types of recombinant baculovirus production and protein expression |
| Sf21 (Spodoptera frugiperda) | High yield of intracellular protein | Intracellular recombinant baculovirus production and protein expression |
| High Five (Trichoplusia ni) | High yield of secreted protein, shorter culture period | Secreted protein expression |
Creative BioMart has more than 10 years of experience in protein expression and is competent to satisfy various protein expression desire. We are expert in protein expression (intracellular or secretory), signal peptide selection, insect cell selection and culture. Choosing our Multi Gene Expression Service, customers only need to provide the coding sequence of your target protein. We will provide customizes service from codon optimization to large-scale expression and purification. For more assistance, please contact us for help.