Polyhedrin starts to accumulate in the insect cell during the late phase of infection to embed the occlusion derived virus (ODV) to form occlusion body. The polyhedrin is so abundant in the infected cells that it accounts for almost 50% of total protein. As polyhedrin is not essential for virus survival, it is wise to use polyhedrin to realize the high-level foreign protein display.
The foreign fragment was inserted into polyhedrin through routine procedure and displayed on the occlusion body. The location of the protein display is shown in the figure.
Our customers usually require our occlusion body surface display service for the purposes of
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| Baculovirus Species | Insertion Site on Polyhedrin | Description |
| AcMNPV | N-terminal 19-110, 32-110 amino acids | Folds of increase in protein yield |
| Amino acid 58-110 | Minimal sequence for incorporation | |
| BmNPV | N-terminal 50 amino acids | Necessary recognition signal length |
| N-terminal 100 amino acids | Most efficient incorporation |
Creative BioMart provides several surface display services corresponding to 5 kinds of infusion strategies. Please click the links below to learn more about our services if you are interested in. If you have any problem, please feel free to contact us.
Use the GP64 glycoprotein as the fusion partner to display the intended protein.
Fuse the foreign protein with VP39 nucleocapsid protein (rather than GP64) and display it on the surface of baculovirus capsid
Adopt the GP64 related strategy to display the protein to the host insect cells
Complete the GP64-related surface display in the non-lepidopteran cell with the help of appropriate promoters