Vector Gallery

As a company with more than 10 years of experience in molecular biology services, Creative BioMart provides a very comprehensive baculovirus expression service. Based on three commonly used baculovirus platforms (AcMNPV, BmNPV, HyNPV), dozens of recombinant protein expression approaches with different advantages can be found in Creative BioMart. In addition to expression services, we also provide corresponding products. Customers can purchase products of interest from us and complete the expression of target protein in their own laboratory with our assistance.

The core of baculovirus expression system is the vector. The characteristics of different vectors determine the characteristics of expression approach. The in our vector gallery, all the vectors corresponding to our available expression approaches can be found. For more information, please feel free to inquire us.

AcMNPV Platform

Donor Vector

Corresponding Approach	Donor Vector	Advantage
Bac-to-Bac Expression Approach	pFastBac 1	Offers strong polyhedrin promoter for high level, native protein expression Large multiple cloning site for simplified cloning Suitable for high-level protein expression
	pFastBac HT	Offers strong polyhedrin promoter Has an N-terminal 6xHis tag for sample purification Has a TEV protease cleavage site for tag removal Suitable for secreted protein
	pFastBac Dual	Contains two strong promoters Able to conduct simultaneous expression of two protein in insect Suitable for simultaneous expression of two protein in insect cell cell
Bac-to-Bac TOPO Expression Approach	pFastBac CT-TOPO	Offers strong polyhedrin promoter Has a C-terminal 6xHis tag for sample purification Has a TEV protease cleavage site for tag removal Suitable for high-level protein expression Allow C-terminal intercellular polyhistidine fusion proteins
Bac-to-Bac TOPO Expression Approach	pFastBac NT-TOPO	Offers strong polyhedrin promoter Has an N-terminal 6xHis tag for sample purification Has a TEV protease cleavage site for tag removal Suitable for secreted protein Allow N-terminal intercellular polyhistidine fusion proteins

Transfer Vector

Corresponding Approach	Transfer Vector	Advantage
BacMagic Expression Approach	BacMagic-1 DNA	Deleted chitinase gene (chi A) High efficiency of secretion protein production High level secretion or membrane location protein production
	BacMagic-2 DNA	Deleted chitinase gene (chi A) Delate cathepsin-like cysteine protease gene (v-cath) High quality and yield for most protein High quality and yield expression for most target protein
	BacMagic-2 DNA	Deleted chitinase gene (chi A) Delate cathepsin-like cysteine protease gene (v-cath) Delate virus genes P10, p24 and P26 Further improved quality and yield expression for most target protein
InsectDirect Expression Approach	pIEx	Has hr5 enhancer and ie1 promoter Can direct protein expression in insect cells For high level protein expression For expression screening
	pBiEx	Has hr5 enhancer and ie1 promoter Has T7 lac promoter Can express protein in both insect cell and E. coli For high level protein expression Protein expression in E. coli and insect cell
	pIEx/Bac	Has hr5 enhancer and ie1 promoter for plasmid-mediated expression Has p10 promoter for baculovirus expression Can express protein in both insect cell and E. coli For high level protein expression Suitable for InsectDirect system and BacMagic system
BacPAK6 Expression Approach	pBacPAK8/9	Contains a strong polyhedrin prompter. Suitable for high-level protein expression. The sequence direction of MCS of pBacPAK8 and 9 is opposite
	pBacPAK8-Nterm 6xHN	Contains a strong polyhedrin prompter. Suitable for high-level protein expression. Frame the IMCA tag at N-terminal of the PCR product Suitable for TALON resin
	pBacPAK8-Cterm 6xHN	Contains a strong polyhedrin prompter. Suitable for high-level protein expression. Frame the IMCA tag at C-terminal of the PCR product Suitable for TALON resin
	pBacPAK8-GUS	Positive control of pBacPAK8/9 Used to monitor transfection efficiency and virus production
	pBacPAK8-Nterm 6xHN-GUS	Positive control of pBacPAK8-N/Cterm 6xHN Used to monitor transfection efficiency and virus production
BaculoGold Expression Approach	pENTR/ TEV /D-TOPO	Directional TOPO cloning 5’ TEV sequence for N-terminal tag removal
	pENTR/ D-TOPO	Directional TOPO cloning
	pENTR/ SD /D-TOPO	Directional TOPO cloning Vector includes the Shine-Dalgarno sequence for E. coli expression-ready entry clone

Entry Vector

Corresponding Approach	Donor Vector	Advantage
BaculoDirect Expression Approach	pFastBac 1	Offers strong polyhedrin promoter for high level, native protein expression Large multiple cloning site for simplified cloning Suitable for high-level protein expression
	pFastBac HT	Offers strong polyhedrin promoter Has an N-terminal 6xHis tag for sample purification Has a TEV protease cleavage site for tag removal Suitable for secreted protein
	pFastBac Dual	Contains two strong promoters Able to conduct simultaneous expression of two protein in insect Suitable for simultaneous expression of two protein in insect cell cell

HyNPV Platform

Entry Vector

Corresponding Approach	Donor Vector	Advantage
BaculoDirect Expression Approach	pFastBac 1	Offers strong polyhedrin promoter for high level, native protein expression Large multiple cloning site for simplified cloning Suitable for high-level protein expression
	pFastBac HT	Offers strong polyhedrin promoter Has an N-terminal 6xHis tag for sample purification Has a TEV protease cleavage site for tag removal Suitable for secreted protein
	pFastBac Dual	Contains two strong promoters Able to conduct simultaneous expression of two protein in insect Suitable for simultaneous expression of two protein in insect cell cell

BmNPV Platform

Corresponding Approach	Donor Vector	Advantage
BaculoDirect Expression Approach	pFastBac 1	Offers strong polyhedrin promoter for high level, native protein expression Large multiple cloning site for simplified cloning Suitable for high-level protein expression
	pFastBac HT	Offers strong polyhedrin promoter Has an N-terminal 6xHis tag for sample purification Has a TEV protease cleavage site for tag removal Suitable for secreted protein
	pFastBac Dual	Contains two strong promoters Able to conduct simultaneous expression of two protein in insect Suitable for simultaneous expression of two protein in insect cell cell

Please note that all products/services provided are for research use only. Not intended for any clinical use.