VLPs have high safety while they can induce immune response, which makes VLPs a powerful tool for vaccine development. With the progress of protein biology, VLP is being applied in more and more fields besides as vaccine. With the expansion of application fields, the composition and structure of VLPs subunits are becoming more and more complex. Baculovirus expression system can accommodate large foreign fragments and perform excellent post-translational modification, so it is regarded as one of the ideal expression systems for VLPs construction.
For customers who want to expression their VLPs which contains 4 or more subunits, please consider Creative BioMart's Multigene Expression Based VLPs Expression Service.
Application of the Multigene Expression Based VLPs Expression Approach
When trying to express a VLP with complex structure, the traditional baculovirus expression vectors usually do not support the insertion of multiple genes or the insertion process is complex.
In order to solve this problem, Creative BioMart provides a VLPs expression pathway and corresponding services that support the simultaneous expression of multiple subunits. Multiple subunit fragments can be inserted into the vector by repeated and simple operations (The two insertion logics is shown in Figure 2). Finally, all the subunits gene cassettes will be expressed at the same time in the host cells. For the detail construction process of the MultiBac expression approach please click here.
Figure 2. The Insertion Logic of the Multi Gene Expression Based VLPs Expression Pathway
| Step | Service Description | Timeline | Deliverables | |
| 1 | System Selection |
|
—— | —— |
| 2 | Generating vectors contains multi subunits gene cassettes |
|
2-3 weeks |
|
| 3 | Preparation of recombinant Bacmid |
|
1 week |
|
| 4 | Recombinant Baculovirus Production |
|
2 weeks |
|
| 5 | Small-Scale Expression & Purification |
|
1.5 weeks |
|
| 6 | Optional Services |
|
1-2 weeks |
|
| 7 | Large-Scale VLPs Expression & Purification (optional) |
|
1-3 weeks |
|
Creative BioMart has more than 10 years of experience in protein expression and is competent to satisfy various protein expression desire. We are expert in protein expression (intracellular or secretory), signal peptide selection, insect cell selection and culture. Choosing our expression service, customers only need to provide the coding sequence of your target protein. We will provide customizes service from codon optimization to large-scale expression and purification. For more assistance, please contact us for help.