Gene Sequence Design and Optimization Service

Decades of experience in gene synthesis has made Creative BioMart an expert in DNA optimization. Creative BioMart fully understands the importance of accurate gene design for its subsequent expression, especially in heterogeneous systems like Baculovirus Expression System.

The optimal expression of gene is realized by systematic design of gene itself, vector, host system and culture conditions. However, when establishing a protein expression approach, scientific researchers typically focus on the selection of appropriate expression vector and host system, rather than consider whether the target gene sequence itself is matched with the vector and system. In the section of sequence optimization, codons optimization, signal peptide and tag adding service are offered to improve the future expression efficiency and stability of target DNA.

CODONS OPTIMIZATION

Codon optimization refers to the redesign of genes by using preferred codons which avoiding rare codons with low utilization, simplifying the secondary structure of mRNA transcription, removing motifs that are not conducive to efficient expression, adding motifs that are conducive to efficient expression, and adjusting GC content. For the codon optimization, we mainaly focus on the following aspects

• Codon Usage Bias

Adjust rare codon components. Combining high-frequency and sub high-frequency codons to make the codon adaptation index (Cai) reach Cai ≥ 0.80 (which is the theoretical high-efficiency protein expression standard).

• mRNA Secondary Structure

Identify and reduce hairpin structure.

• Removal of Trans Acting Elements and Restriction Sites Affecting Expression

Selective deletion of restriction sites in order to avoid the impact of site conflict on the construction of expression vector.

• GC Content

GC content directly affects the binding stability and annealing temperature of DNA sequences

• Translation Start and End Efficiency

Effective translation termination can promote protein expression.

• Custom Modifications

For example, ribosome binding site modifications and RNA motif alterations

If you have any special needs beyond the list above, please feel free to contact us for more details. We are always willing to exchange optimization solution with you to achieve satisfactory results.

SIGNAL PEPTIDE AND TAG ADDING

In response to protein secretion, purification and other requirements, we can add signal peptide or protein tag to the target sequence.

If you need to fuse your DNA sequence with other tag protein sequences, please contact us for more details.