After the small-scale expression of the recombinant protein, qualitative assay of the expressed product is required to confirm that the target protein has been successfully expressed. Two protein qualitative assay methods are available at Creative BioMart.
WB is a commonly used techniques for not only qualitative but also quantitative assay of protein expression. This technology is based on the combination of (1) immunobiochemistry technology; (2) gel electrophoresis and; (3) solid-phase immunoassay technology.
Application
The method has the advantages of fast identification, simple operation, clear results and easy analysis. Comparing the protein size corresponding to the target protein band with the theoretical size, researchers can preliminarily confirm whether the protein is successfully expressed. At the same time, the protein yield can also be roughly determined according to the band width. The results are intuitive and suitable for the preliminary identification of proteins.
However, this method can only reflect the concentration and size of protein. In order to evaluate whether the target protein is expressed successfully, it is necessary to check the parameters such as post-translational modification. Customers who want to accurately check the target protein, please consider our De novo protein sequencing.
To evaluate whether a recombinant protein is correctly expressed, it is necessary to check not only whether the amino acids form the peptide chain as expected, but also the peptide chain folding and post-translational modification.
De novo sequencing is a relatively new method. It can realize accurate sequencing of amino acid sequences independent of protein database and can check post translation modification. It is very suitable for the detection of modified new proteins.
For customers who have established a mature expression system, western blot can fully meet your testing requirements. Customers can save their cost by choosing this simple detection method.
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