InsectDirect System

A rapid, virus-free expression service is available in Creative BioMart. Customers who are looking for technical support on determining the optimal expression system for multiple target protein please consider our InsectDirect expression service. The plasmid-mediate expression approach can rapidly direct milligram-scale target protein expression in insect cells without undertaking the time-intensive step like virus tittering and amplifying. On top of the simplified expression approach, a set of high-efficiency transfection and purification method is also applied to facilitate the service. As a result, target protein can be harvested in 48 hours after transection.

InsectDirect System

InsectDirect system is a virus free system which mainly involves a transfer vector and a host insect cell (Figure 1). The transfer vector is framed with a hr5 enhancer and an immediate early promoter ie1 thus can direct induce the transcription in host insect cells. Thus, the step of construction recombinant virus, plaque assay, virus tittering, and amplifying can be eliminated in InsectDirect. The InsectDirect system exhibits the feature including,

An initial pilot method to determine best expression ingredient
Allow high throughput screening of multiple target protein
Allow protein expression within 48 hours
Allow scale up by enlarging the culture volumes
Offers several types of vectors to meet different expression requirements

InsectDirect System

Service detail of InsectDirect System

Here is the service process. For more technical information or enquiry, please feel free to contact us.

Step		Service Description	Timeline	Deliverables
1	System Selection （Optional）	Select transfer vector (pIEx, pBiEx) Select host insect cell line (Sf9, Sf21, High Five)	——	——
2	Prepare Target Gene	Target gene sequence design and codon optimization Gene synthesis Subclone the interest sequence to the transfer vector	< 1week	Enzymatic / Sequencing validation report.
3	Transfection	Transfect the insect cell with recombinant transfer vector	2 days	Small volumes of recombinant protein
4	Recombinant Protein Process	Product qualification Recombinant purification and tag removal	< 1week	Recombinant protein QC report
5	Optional Services	System optimization Activity assay Scale-up expression	1-2 weeks	Optimization solution Protein product Protein activity report

Transfer Vector and Insect Cell Line

Transfer Vector

The expression level of pIEx is slightly higher than the other ones. Thereby, pIEx transfer vector is more recommended for high-level expression.

Transfer Vector	Feature	Use
pIEx	Has hr5 enhancer and ie1 promoter Can direct protein expression in insect cells	For high level protein expression For expression screening
pBiEx	Has hr5 enhancer and ie1 promoter Has T7 lac promoter Can express protein in both insect cell and E. coli	For high level protein expression Protein expression in E. coli and insect cell
pIEx/Bac	Has hr5 enhancer and ie1 promoter for plasmid-mediated expression Has p10 promoter for baculovirus expression Can express protein in both insect cell and E. coli	For high level protein expression Suitable for InsectDirect system and BacMagic system

Creative BioMart offers vector with various function. We have N/C-terminal fusion tag vector, tag-free vector and LIC-prepared vectors under each category of transfer vector (pIEx, pBiEx, pIEx/Bac). Please click here to see our vector gallery and get the guidance of vector selection.

Recommended Insect Cell Line

Sf9, Sf21, S2, and High Five cells are all suitable for the InsectDirect system while the best result can always be obtained with Sf9, Sf21 cell line. Please click here to learn more about the feature and use of the host insect cell lines.

Creative BioMart has more than 10 years of experience in protein expression and is competent to satisfy various protein expression desire. We are expert in protein expression (intracellular or secretory), signal peptide selection, insect cell selection and culture. Choosing our comprehensive service, customers only need to provide the coding sequence of your target protein. We will provide customizes service from codon optimization to large-scale expression and purification.

Please note that all products/services provided are for research use only. Not intended for any clinical use.