Baculovirus Capsid Surface Display Service

Except for fusing with GP64 protein, the foreign can also be surface displayed through fusion with nucleocapsid protein. VP39 is the major nucleocapsid protein in AcMNPV. The VP39 protein monomer is lined around the stacked rings of baculovirus nucleoprotein core abundantly. The protein of interest can be displayed along the capsid surface by fusing them to VP39.

Service Description

The foreign fragment was inserted into VP39 through routine procedure and displayed on the capsid of the virus. The location of the protein display is shown in the figure.

Technology Applications

 Our customers usually require our capsid display service for the purposes of  

• Study the transduction route of baculovirus in mammalian cells from the cell surface into the nucleus
• Elucidate the internalization and intracellular trafcking pathways of baculovirus in both insect and vertebrate cells
• Other imaging studies
• Enable nuclear delivery of therapeutic proteins to achieve the desired treatment efects as efectively as current protein transduction methods

Technology Advantages

Compared with other fusion strategies, the capsid display exhibit the advantages of

• The protein is only displayed around the stacked ring thus less interference for virus assembly
• High levels of protein display due to the abundant VP39 protein amount
• The display uniformly distributed along the surface
• Large capacity—— 41 KD or even larger protein is able to be displayed
• Ability fo accommodate both N- and C- terminal protein fusions due to the flexibility of capsid protein.

What Related Services Creative BioMart Provides for Our Customers

If you have any questions or are interested in our service or if you have other forms of integration needs, please contact us. Our professional technical team will wholeheartedly provide customized solutions for you.

• GP64 glycoprotein-related surface display service

Use the GP64 glycoprotein as the fusion partner to display the intended protein.

• Occlusion body surface display (OBD) service

Surface display of foreign proteins using baculoviral polyhedrin as a fusion partner

• Infected insect cells surface display service

Adopt the GP64 related strategy to display the protein to the host insect cells

• Mammalian and other non-lepidopteran cell surface display service

Complete the GP64-related surface display in the non-lepidopteran cell with the help of appropriate promoters