Protein separation and purification is an important part of proteomics research. Creative BioMart has been committed to the related research of protein expression and purification for many years. With comprehensive protein purification technology and various purification systems, Creative BioMart can provide diversified protein purification schemes, as well as large-scale, scanning type protein purification and separation work, helping save precious research time and get different needs met. We can provide you with customized protein purification services, including tag / unlabeled recombinant protein, purification and separation of natural protein, etc. According to the properties of protein, we design many kinds of protein purification experimental schemes, such as affinity purification, ion exchange, molecular sieve, and hydrophobic. The obtained high-purity protein can be customized point-to-point according to customer requirements, which can meet a series of fine experimental requirements such as protein folding and recrystallization.
The commonly used separation and purification methods include membrane separation technology, precipitation separation technology, electrophoresis separation technology, and chromatography separation technology. Among them, membrane separation technology and precipitation separation technology belong to crude protein extraction, and electrophoresis separation technology is mainly used for detection and analysis. At present, chromatographic separation technology is mainly used for protein purification, which is usually divided into the following methods.
| Purification Method | Principle | Scope of application |
| Gel filtration | Separation depends on the size or shape of the molecules | Macromolecular compounds with large molecular weight |
| Ion exchange | According to the difference of surface electrostatic charge | Mainly for proteins with isoelectric point greater than 5 |
| Hydrophobic | Based on protein hydrophobic interaction different | Can be used in the purification of all proteins in principle |
| Affinity | According to affinity between bioactive substances ligands | Suitable for antigen antibody affinity purification and label purification. |
| Project Name | Purification Scheme |
| Label removal and purification | The fusion protein containing 6xHis, GST, sumo and MBP was isolated and purified by commercial affinity purification resin, such as Ni column and GST beads. The purity of fusion protein was more than 90% |
| Tag less protein purification | Antibody affinity purification, or similar to natural protein purification, depending on the project |
| Denaturation and renaturation of inclusion bodies | Depends to the different properties of proteins. Urea, guanidine hydrochloride, detergents, extreme pH can be selected to ensure the monomer soluble protein. |
Step 1
Protein detection and pre experiment. SDS-PAGE detection of protein; small volume purification and detection
Step2
Protein purification and detection. Mass purification; SDS-PAGE; WB detection (depending on the project); concentration detection
Delivery Materials
Purification report, original picture, protein product.
Service Features
If you are interested or have questions about our service, please contact us.