After the small-scale expression of the recombinant protein, qualitative assay of the expressed product is required to confirm that the target protein has been successfully expressed.
Western blot (WB) is one of the common techniques for qualitative and quantitative assay of protein expression. The technology is a new immunobiochemistry technology developed on the basis of gel electrophoresis and solid-phase immunoassay technology. Therefore, it has the high resolution and high specificity of the two technologies of SDS-PAGE and solid phase immunoassay. It is a convenient and reliable research tool and plays an important role in proteomics with mass spectrometry and protein chip technology.
Western blot experimental service has been mature and widely used. However, it is not easy to get a clear background, bright band, real and objective experimental results, which requires a lot of time and energy.
Creative BioMart has the skills of summarizing experimental operation details, strict quality control points and strict pre experiment settings. We can customize different experimental schemes according to different customers' recombinant protein, then provide clear and complete Western blot experimental results, which can save customers' valuable research time and meet customers' needs with high quality.
Different protein concentration determination methods (Lowry method or BCA method) are adopted for different protein samples.
Internal parameters are used to accurately quantify.
For the same sample, the quantitative value difference between repeated experiments is less than 8%
With years of experience in antibody research, Creative BioMart has rich knowledge of antibody selection, and the false positive results can be avoided.
If you are interested or have questions about our service, please contact us.
Creative BioMart has been devoted to protein expression research for many years. Our scientists have accumulated a wealth of experimental operation experience to ensure the rapid and accurate program completion. Creative BioMart has established a perfect protein assay platform, with precise protein detection equipment and technology, we can provide one-stop technical services from recombinant protein preparation to protein expression and purification, so as to ensure high-quality service content.