GP64 Glycoprotein-Related Surface Display Service

GP64 is the main envelope glycoprotein for group I baculovirus (including AcMNPV and BmNPV). It plays an important role in membrane fusion into the insect cell upon endocytosis. 

The a GP64 sequence basically consists of a transmembrane domain (TMD), a C-terminal cytoplasmic tail domain (CTD) as well as a N-terminal signal peptide (SP). Each domain plays an important role in membrane fusion into the insect cell upon endocytosis. GP64 it is the core of most baculovirus surface display technology.

Our Services

Different forms of GP64 fusion position can be deployed in baculovirus surface-display techniques, which not only affects the viral growth characteristics but also affect the actual presentation of the epitope. As your reliable research assistant, Creative BioMart offers services corresponding to several different GP64 related fusion forms. Customers can make selections according to the actual situation.

Fuse the displayed protein with the entire GP64

The coding sequence of the target protein is inserted into the viral DNA via the standard procedure, producing a "GP64-target protein-full GP64 protein" sequence.

Features

• The protein of interest is fused to the N-terminus of the GP64 membrane protein
• The fusion protein is expressed alongside the WT GP64 on the virion
• Ideal for immune recognition related utilization
• Suitable to be used as immunogens for vaccine or monoclonal antibody production

Fuse the displayed proteins with partial of GP64

The foreign protein coding sequence is inserted and expressed in a fusion form together with either SP, CTD or TMD+CTD domain. Although the integrity of GP64 is destroyed, the expression and display of the target protein can still be realized.

Features

• Can be realized through simpler cloning procedure
• The display effect relies on the properties of the fused protein (size in particular)
• Suitable for shorter target protein display
• The GP64 TMD can be replaced with the heterologous TMD and CTD of vesicular stomatitis virus glycoprotein (VSVG) to improve the display effect
• The fusion protein is expressed alongside the WT GP64 on the virion

Peptide insertions into the native GP64 protein

Features

• The fusion protein is NOT expressed alongside the WT GP64 on the virion
• Have the capacity of peptide sequence that less than 23 amino acid
• A little less infectious and GP64 incorporation compared to virus with WT GP64

If you have any questions or are interested in our service or if you have other forms of integration needs, please contact us. Our professional technical team will wholeheartedly provide customized solutions for you.

• Baculovirus capsid (BCD) surface display service

Fuse the foreign protein with VP39 nucleocapsid protein (rather than GP64) and display it on the surface of baculovirus capsid

• Occlusion body surface display (OBD) service

Surface display of foreign proteins using baculoviral polyhedrin as a fusion partner

• Infected insect cells surface display service

Adopt the GP64 related strategy to display the protein to the host insect cells

• Mammalian and other non-lepidopteran cell surface display service

Complete the GP64-related surface display in the non-lepidopteran cell with the help of appropriate promoters