Mammalian and Other Non-lepidopteran Cell Surface Display Service

The fact that baculovirus can enter other cell types without causing any cytopathic damange makes baculovirus a perfect tool for heterologous modification. Through being inserted at the downstream of some  appropirate promoters (which can be recognized by mammalian cells or other cells), the target sequence can be expressed in cells of other species mediated by baculovirus.

This strategy is the so called "BacMam Expression System". Based on the BacMam system, the gene of interest can also be displayed on the surface of mammalian and other non-lepidopteran cells. This service is suitable for customers who want to express pharmacological cell-based assays for cellular receptors, transporters, ion-channels, and viral targets.

Service Description

Baculoviruses can carry out the protein display in cells of the following organisms: human, rabbit, non-human primate, rodent, porcine, bovine, ovine mammalian cells, from fish and mosquito.

Baculovirus-mediated surface display greatly relies on the interaction of the virus GP64 with mammalian surface phospholipids. The target gene was inserted into the full-length or part of GP64 expression cassette, meanwhile there is an upstream CMV or SV40 promoter which could be recognized by mammalian cells. In this way, the foreign gene expressed in mammalian cells will be guided and anchored in mammalian cell membrane.

Creative BioMart provides three forms of fusion options for customers to choose from.

• Solution 1: Cloning the foreign protein coding sequence into the expression cassette with its full-length coding region which includes signal peptide and transmembrane domain
• Solution 2: Cloning the sequence into a truncated GP64-fused form
• Solution 3: Cloning the sequence into a truncated form fused to foreign anchor domains
• Solution 4: Cloning the sequence into a truncated GP64 which contains VSVG

The foreign fragment was inserted into polyhedrin through routine procedure and displayed on the occlusion body. The location of the protein display is shown in the figure.

What Related Services Creative BioMart Provides for Our Customers

Creative BioMart provides several surface display services corresponding to 5 kinds of infusion strategies. Please click the links below to learn more about our services if you are interested in. If you have any problem, please feel free to contact us.

• GP64 glycoprotein-related surface display service

Use the GP64 glycoprotein as the fusion partner to display the intended protein.

• Baculovirus capsid (BCD) surface display service

Fuse the foreign protein with VP39 nucleocapsid protein (rather than GP64) and display it on the surface of baculovirus capsid

• Occlusion body surface display (OBD) service

Surface display of foreign proteins using baculoviral polyhedrin as a fusion partner

• Infected insect cells surface display service

Adopt the GP64 related strategy to display the protein to the host insect cells