Infected Insect Cells Surface Display Service

Compared with other expression systems, insect cell has the advantages of simpler culture conditions, ability to perform post-translational modifications and ability to perform correct protein fold. Consequently, except for budded virus and occlusion body, the foreign protein will also be displayed on the surface of infected insect cells.

Service Description

GP64 and GP64 fusion proteins will be transferred to the infected cell membrane before the virus particles sprout. As long as the foreign protein can be bound to a membrane-bound protein with appropriate SP and TMD, the intended protein can be expressed together with the membrane binding protein and translocated to the surface of host cells. To do this Creative BioMart provides three fusion options for customers to choose from.

• Solution 1: Cloning the foreign protein coding sequence into the expression cassette with its full-length coding region which includes signal peptide and transmembrane domain
• Solution 2: Cloning the sequence into a truncated GP64-fused form
• Solution 3: Cloning the sequence into a truncated form fused to foreign anchor domains

We usually recommend inserting the target protein into the N-terminal signal peptide and C-terminal membrane anchoring region (solution 2), because doing so can increase the expression of the fusion protein on the cell surface. This truncated construct is much smaller than that containing full-length GP64, which reduces the probability of being cleaved by protease when it is delivered to the cell surface.

The foreign fragment was inserted into polyhedrin through routine procedure and displayed on the occlusion body. The location of the protein display is shown in the figure.

Technology Applications

Our customers usually require our occlusion body surface display service for the purposes of  

• Over-expression of pharmaceutically important membrane proteins for functional studies and screening of interacting molecules
• Over expresse the protein that are expressed in low amounts in mammalian cells
• Protein function study
• Study the pharmacological properties of G protein-coupled receptors

What Related Services Creative BioMart Provides for Our Customers

Creative BioMart provides several surface display services corresponding to 5 kinds of infusion strategies. Please click the links below to learn more about our services if you are interested in. If you have any problem, please feel free to contact us.

• GP64 glycoprotein-related surface display service

Use the GP64 glycoprotein as the fusion partner to display the intended protein.

• Baculovirus capsid (BCD) surface display service

Fuse the foreign protein with VP39 nucleocapsid protein (rather than GP64) and display it on the surface of baculovirus capsid

• Occlusion body surface display (OBD) service

Surface display of foreign proteins using baculoviral polyhedrin as a fusion partner

• Mammalian and other non-lepidopteran cell surface display service

Complete the GP64-related surface display in the non-lepidopteran cell with the help of appropriate promoters