Protein quantitation is often necessary before processing protein samples for isolation, separation and analysis by chromatographic, electrophoretic and immunochemical techniques. Depending on the accuracy required and the amount and purity of the protein available, different methods are appropriate for determining protein concentration.
Here are some quantitation methods available at Creative BioMart. But please note that no method is perfect for every protein. The advantages and disadvantages are listed below. Customers can choose according to the instructions or contact us for help.
UV absorption
Simplest and most direct assay method for protein concentration determination
Biuret methods:
Protein-copper chelation and secondary detection of reduced copper
Colorimetric dye-based methods:
Protein-dye binding and direct detection of the color change
Fluorescent dye methods:
Protein-dye binding and direct detection of increase in fluorescence associated with the bound dye
Assays can be adapted for automated handling in high-throughput applications due to less significance of timing
Creative BioMart has been committed to protein expression, purification and analysis for many years, and has established an advanced technology platform. Our professional technical team can provide appropriate protein sequence services according to the protein provided by customers.